The presenters developed and implemented a semester-long, student-centered, discovery-based laboratory research experience that actively engages students in the process and application of science. Screening local insect populations for novel strains of the bacterial endosymbiont Wolbachia provides the framework for students to develop competencies in the following: quantitative analysis, technical skills, scientific communication, and information literacy. According to survey data, students express an increase in confidence in these four competencies. Objective assessments indicate student gains in understanding molecular biology concepts and skills as well as the interdisciplinary nature of science. These data indicate achievement of project goals. The transformation of our introductory lab course has challenged students to develop critical thinking skills that should prepare them for success in STEM and other academic fields.